FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15
Sequences flagged as poor quality0
Sequence length75
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAACACCCGACGGCGCAGACGACAAACTCGAGGCACTTAATCACAGCGAGCTGATCCCACTAGCAAACTACG16.666666666666667No Hit
GGAGTTCGGTGCTCCCTTCCATCCAAGAAAACTGTGTTGTCTTCTCCGAAGCCAACCACCGATCCAACATAGAAA16.666666666666667No Hit
CCTGACATTGGCTTCGCCCGCGAATTAGCCAAATCTGTGCACGCCAAGTGCCCCGGCTAGCTGCTGTCGTACAAC16.666666666666667No Hit
GCGCCGGGAGACGTGTGCTCTTACGATCTGAACCGGGAGACGCGTTCTCTTAGTAACAGAACGGGCGGACGTGTC16.666666666666667No Hit
CGTCTCCCTGAGCAGATCGGAAGAGCACACGTCTCCCAGAGCAGATCGGAACAGCACACGTCTCCCCTGTCTCTT16.666666666666667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTGCTCTTCCGATCTGGTGTGGGAGACGTGTGGTCTACCGATCGCGACAGGGACACGTCTGCACTTCGGAACA16.666666666666667No Hit
GCTCTTCCGATCTTGTCTGGGGCTCTTCCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGT16.666666666666667No Hit
CTTCCGATCTTTTTTTTTTTTTATTATTATTATTTTTTAGAAAGACTACCCCGCGTCTAAAGGGGAGACAAAGCC16.666666666666667No Hit
GTCTCGAAGGGGCAGCGCGCGGTGGTCGCGGGCGACCTGCTGCCCCACGCGATCCAGTGCCGCGAGCCGGCGTGG16.666666666666667No Hit
ATTCTCCTGCCTCAGCCTCCAGAGTAGATGGGACTACAATAACTTAGTAATTCTATAACATAAAGTCAAATACAC16.666666666666667No Hit
GTTGGAGATCGGAAGAGCACTCGTCTCCCTGTCTCGTATTCAATTCTACACTTCTAACTGACGAGAACGTAGGAA16.666666666666667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATTCATACTGCCTCCTTTCGAGAGCAGCAACATTGCACACACTCCCTACAAACCGGAAGGTGCGGTGCCCAGAA16.666666666666667No Hit
TCCGATCTCTCACCAGCGACACGGCCACCAGCACCGAGGCAACCCCTGCCCTGAACCGGCTCCCCATCACCCATG16.666666666666667No Hit
CCTTCCTGGTTGATCTCCAGAACCAGCTCGGGATCCGCCAGCTCCGGCAAGTCTGCCCCGTACATGCGCTTCACT16.666666666666667No Hit
CCCAACTCAGATCGGAATAGCACACGTCTACCTACACACATCGGAAGAGCACCTGTCTCATTGGAAAATAGGGGG16.666666666666667Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)