FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTACTCCTCCGTTACTCTTTAGGAGGAGACAGCCCCAGTCAAACTACCCACCATACATTGTCCTAGGTTTTT15.88235294117647No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGATGAGAAGAGAACAGAAGAGAAGAGAAGAGAAGA15.88235294117647No Hit
CCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTC15.88235294117647No Hit
GAGTAGCAGTGCCCGCCCAGCCTTTTTTCCGATCGCACTCGCGCTCCTGCACCGTCCCGTCTGCAGTCGTGTAGG15.88235294117647No Hit
GGACTACGAAGCCGCCGCAGCCTTCCCAATCTCCGTCAGCAAAACAACCGCCTCAGCGAAATTGTCCTGCACAAT15.88235294117647No Hit
GTGTACTTCGCTAGATTCACCAGCATACCTGACTATCCTGTTTAGCCGCACACATGCCTCTAAACTGATACTAAT15.88235294117647No Hit
ATTCCGGAGAGGGAGCCAGAGAAACGGATACCACATCTAAGGAAGCCAGCAGGCGCGTAAATTACCCACTCCCAG15.88235294117647No Hit
GTATTACGATATCATACATAAAGGTATAGTACTAACGACATTAGAAACATGTACAACCCGTAAGAAGCTCAAGTT15.88235294117647No Hit
CAAATTTAATTAAAAGATATCTACAGGATTATGGAGCTGAAGCTTATCTAGCCTTCATTCATGTTCGCAGTAAAA15.88235294117647No Hit
CCTGAATATTCTGTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAA15.88235294117647No Hit
CATATATATATATGCTGAGAAATGCCGTTGCCAGCAGCCGCTGCAGCAACAACAGCAATGGGTAACCATTTGAGA15.88235294117647No Hit
CTCTTGTTGTGGTGTGCATTCGTGTGTGGGCAAGTGGCTGTGTGTGTGGGAGTGTAGCAGTGTGGGTGTTTAATA15.88235294117647No Hit
ATATCAATTCATTCCCGATTTTTGTCAATGGACCTATTGTAATAATCATCAAAATTCCATAAATTTAATCGCCTC15.88235294117647No Hit
ACCAAAAACAACGTGACCACGCACCTACATCTACATATAAAACCCCCAAAAAGTGACACGCCCAATATCAATCGA15.88235294117647No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGACCACAAGTGAAAAAAAGAAAAGAGAGGA15.88235294117647No Hit
TCGTGGTACGGCACCAGTAATTTTAGGCAAGACTAAGTTTATCTTTGTGTCCCGACGTCCGATCGGACTTCTGTC15.88235294117647No Hit
GTTATAAAAGAGCATCAGATCTTGCCCCCGCGCACGCAACCGCCCCGCGCATAACGAATCGACCCTGACCAGAGA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)