FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGTCAGCCCGACGGCTGCGTTCAACTTAAAACGGACTTGTGTTCTTAATTGCAGTACATCATTACATGGC14.3478260869565215No Hit
GAGAGAGAGAGAGAAGAGAGAGAGAAGAGAGACAGATAATAGCTAGATATCAGAGAGCAAAACAAAAGCTCATAG14.3478260869565215No Hit
TGCAACGGGAGACGTGTGCTCTTCCTATCTCAAAAGGCATACTACGCCTCTACCCATCACACACGGAAGACGTGT14.3478260869565215Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCATGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAA14.3478260869565215No Hit
GGGCATGGGATAATGAAATAAGACCTGTGTTCTGCTTTCAATTGTTTACAGATCAAGACGTAATGAAAAATAGAA14.3478260869565215No Hit
CCTCTAACTTTCGTTGTTGATTAATGAAAACATCTTTGGCAAATGCTTTCGCTTAAGTTAGTCTTACGACGGTCC14.3478260869565215No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTATAAGTACACACGAATTAAAAGTGAAACCGAAAAAGGCT14.3478260869565215No Hit
GATTCTGACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTTCGCGCCACTGGCTTTTCAACCAAGCGC14.3478260869565215No Hit
CTTACATAAATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAACTTTAAATTTAAAGATATTGCTTTATAACGTTAT14.3478260869565215No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGATCTAGATTAATGAATACATCTTTGGCAAAAGCAATCGCTAAA14.3478260869565215No Hit
GAATCACAGGGCGTAAAGGGCGCGTAGGCGGCCGATTAAGTCGGGGGTGAAAGCCTGTGGCTCAACCACAGATTT14.3478260869565215No Hit
ATCTTCCTGCTATTTAAAAACCTGCATTAGTCCTCTTAAACGAGTGATATTGTGTGCCGGTACTATTACTTTGAA14.3478260869565215No Hit
GAGTTAAAGGTAGGACCTCAAAGTATAACAATCTTGAAAGACACCCTAGAAAACACTCTTCTCAACATCAGCCAT14.3478260869565215No Hit
ACCAATATAACGCTCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCCCATAAGGTTCAAGTTTTAAT14.3478260869565215No Hit
ATCATCGCCTTGGCGATTTCCTCGGTCGCCTTCGACCAGGCGTCGACGACCTTGTTGTACTTCTCGCCGTGGGTG14.3478260869565215No Hit
CTTCGTATGATATCCAACCGCACTTCAACCAGAAAACTCCTCCCCCAGCTGAGCCCCCTCCCGAGTACCTCGACG14.3478260869565215No Hit
GTACGTAATAACATTCATTGTGGATGCTCCCTTCAATAGAAATAACCGTACTGCGAAAATGTCAGTTCGGTGGAT14.3478260869565215No Hit
TATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCC14.3478260869565215No Hit
ACCCCTGACGTCTATAATCTCGGTAAAGCTGTTGGAGGCGGCGTTATCCCTGTCTCTTATACAAATCACCGAGCC14.3478260869565215No Hit
CGCTTAAGTTAGTCTTACGACGGTCCAAGACTTTCACATCTCGAGTCTTCATACTACTGGCCACAAAATGCATCT14.3478260869565215No Hit
ACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGAT14.3478260869565215No Hit
TAGCTATTATTCCAGCTACTTTATTAGCGCAAGGACAAGTGAAAATTGACGGATTACCGCAAATCTCAGATGTTG14.3478260869565215No Hit
ATACACATCTCCGAGCCCACGAGACACTCGGGCATCGCGTTTGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)