FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCTTGCTCTGTCCCCCAGGCTGAAGTGAAATGGTGCCATCTCGGCTCAAGGCAACCTCTCCCACCTGGGTTC18.333333333333332No Hit
TTCCAATTACAGGGCCTCGGATATGAGTCGTGTATTGTTATATTTCGTCACTACCTCCCCGCGCTGGGAGTGGGA18.333333333333332No Hit
CTTCCGATCTGCGTAGGGGTGTCAACAAAAGGCGTCTACGTCAACGCGGATCCTGGGTTGGTGCAACGGGCAAAG18.333333333333332No Hit
ATAGTTGTGTTCGAATTGAGAGTCAAAGTGATGAGAACTGGAGTGACACTTCACGTTACGTTAAGCACATTCAAT18.333333333333332No Hit
GTCTTACGACGGTCATAGAATTTCACCTCTCGCGTCCTAATACTAAAGCACCCAAAATGATTCTATTAATCATTA18.333333333333332No Hit
TCGTATGGTCTTGTACCGACGACAGATCTTTCAAAAGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTA18.333333333333332No Hit
GTACATCACAGTCGAGGAGGAGTGTGACTCTGACGCCTTCGCCTAAGCGGACTCTGATCCGGACTCTGATCCCCA18.333333333333332No Hit
GATTGGAATGGAACAGAATGGAATGGATTGGAATGGAATCGACTCAAATGGAGAGAAACCGAGTGGAATGGAATC18.333333333333332No Hit
GTGTAGCAGAGTGGGTGTTTAATAGAAGGTCTCCTCCTCCAACCAGCCTCCTCCGTTGCGGCGCAAGTAGAAACG18.333333333333332No Hit
GCGATATATAGGGCCGTTCCGTCCAAGTTGATGTTGCATCCGATAGGAAGGCCAGATCGCGTGAACCGCGGATCA18.333333333333332No Hit
TAATTACTGCCATAATGATCGCCATTTATTTGGGTCAGTGAAGTCGTTGTATGCGGCACATAGAGAATCGAATTG18.333333333333332No Hit
GCAATGTCCTCGACGGTCTCGTTGCCCTCGGATATAATACCGACAGACTTGGCGATGGCCTTGGCAGTGATCGGA18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)