FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAAGAGTGGTATTTTCTTCACAACTCGTCTAAAAATAGTCAGATAAGTACAGGAATAACTGTTTTGCACCTA15.88235294117647No Hit
GGTGTCGTCAGCTCCTGTCGTGAGATGTTCGGATAAGTCCCGCAACGAGCGCAACCCATGTCATTAGTTGCTACA15.88235294117647No Hit
CTATTAGCTGGGCTTAATCGTTTTAATCGTCGATTGGCTACATATGAACTTGAATTTAGTGTAGATTGTTGATTT15.88235294117647No Hit
GTATGGTCTTGTACCGACGACAGCTCTTTCAAATGTCTGCCCTATCACCTTTTGATGGTAGTATCTAGGACTACC15.88235294117647No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCACATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAGGGGG15.88235294117647TruSeq Adapter, Index 11 (96% over 26bp)
ACCCGAAGCACCCGCCGATCACGGAAGGTGAGGCCGAGGCCTACCTCGCCCAGGATCTGAAGGTGGCGCTTGCTG15.88235294117647No Hit
TCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCTCTTCTCACATCTCTCTAGACAGGAGAAAATTGCGA15.88235294117647No Hit
NGGTGGTTGTGGTGGCGGTCGTGTACGGGACGGCTGCGCAGCGCGGCCTGGTCAGTGCGGGCCTGGGGCTGGGTG15.88235294117647No Hit
GGCCTGGGTCTTACGATCTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGACAAGACGAAA15.88235294117647No Hit
ATATCTAGAACACTCATAAAATTTATACATATATGAAGCCATCATAAAATCTTAGTGTAGTTTCAAAGAATCAGT15.88235294117647No Hit
CCCGTGCCTCGAGGCATGACAAGGTGGCCGGTATCGATGCAGGGGCCGATGACTATTTGGTCAAGCCATTCCACA15.88235294117647No Hit
CTCTTCTCTCTTCTCTTCTCTCTTCTCTAATCTCTCTTCTCTTCTCTCTTCTCTAATATAATAAATAAAGGGATA15.88235294117647No Hit
GGAATGGAGTAGAGTGGAATGGAATGGAAAGGAACAGAATGTATTGCAAGGTAAAGGAATGAAGTGGAGTGGAGA15.88235294117647No Hit
CGCCTGTTTAATAAAATCACAGCTCTATGCAAACAAGAAAGTCGACTTATAGTCAGTGACTAATGACCGGTGCTG15.88235294117647No Hit
TTGTTATTCTATGCCATTCTATGTTATTCTTTGTTATTGTATGCCTGTCTCTTATTCACAAGTCGGAGCAGTGGT15.88235294117647No Hit
ATATAAATCTGATGTGTTTAGCATGTTTCAAGATTTTCTACCCAACAGTGAAGCTCATTATAATTTTAAGGTTGT15.88235294117647No Hit
ACATGATACTTATTATGTAGTAGCTCATTTTCATTATGTTTTATCTATAGGAGCTGTATTTGCTATTATAGCAGG15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)