FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n01_zt2s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n01_zt2s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATTGCCACGGAGCAACTTGATCTTGCGATCAAAAGAGTAGTTTTTCTGGTTTGAAGATCTCTATGATTTCCA16.25No Hit
GGTATAGTACTAACCACAATAGTAAGTTGTCCTACGCGTATGAAGCACAAGTTCAACTACGAACGTATTAACCGC16.25No Hit
AAAAAAAAGATCGGAAGAGCACACGTCTCCCTCGCGAGATCGGAAGCTGTCTCTTATACACATCTCCGAGCCCAC16.25Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGAAATGTAGTAGATATGAAGATCGAACATGGAACATCAGGGAGCGGTCTGTGTCTCTGTGTCAGTCACCTCTCT16.25No Hit
CGCAACGACAAGTTCGATGTAAACACCTTCCAGGACACCTCCCAGCTGCCCATTCGATCCCGCAAGAAGAATAGC16.25No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATACCACGATACCCGTAGCAACCATGGTAGTCCTAGATACTACCATCAA16.25No Hit
ATTTCAGACACAGTGGCCTGTTCAAATTCTCTGGAATTTAGCCTGGATATTTTCTCTTGCATCAGGACAGTGTTA16.25No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGCTAATGATTAATAGCTGTCTCTT16.25No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16.25No Hit
GGAGACGTGTGCTCTTCCGATCTAGCCCGGGAGACGTTTCCTCTTCATATCACCACCGGGACACGAGTCATCGTA16.25Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTGTGCTTCATACGGGTATTACAACTTACAATAGTGTTTAGTACTATACATGTCTCTTATACACATATCATA16.25No Hit
GCAGTGAAACTATGTGAGTTTCTTGGCCCACTTCAGGTCGACATCGCGGGGCTTGAGGCCCGTAAAATGCTCGAT16.25No Hit
ATATTATTCTCTTGCTCCAGCCTCTGCTAGCCACAGAATTGCACGTAGATATCTCAATCATTTGATGACTTTTTT16.25No Hit
AGCTAATACATGCAATTAAAACATGAACCTTATGGGACATCTGCTTTTATAAGGCTAAAACCAAGCGATCGCAAG16.25No Hit
GATCGAAGACGTCAAGGAGATGTACTAAGGCATCACCTACCTTATCAAGCTCTAGAAGAGACTGTAAATTGCACG16.25No Hit
GTGTTTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCATTATAAGGACACTAGCTTCTTAAATGGACAAATTG16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers