FastQCFastQC Report
Tue 5 Nov 2019
HW2VHAFXY_n02_14hCut_9h14h_Oct25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2VHAFXY_n02_14hCut_9h14h_Oct25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183739610
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5311570.28908137989407945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6779100.065.454831
AGCAGTG6804100.064.995642
GCAGTGG7885400.056.0033953
AGTGGTA8612850.051.275185
CAGTGGT9156800.048.257054
GTGGTAT9789900.045.0764966
GGTATCA9947700.044.3234638
TGGTATC10162500.043.4260677
GTATCAA10279100.042.8386469
CAACGCA9791000.025.58901612-13
ACGCAGA9809500.025.54044514-15
ATCAACG9984400.025.13314410-11
GCAGAGT10048100.024.93089516-17
AGTACAT9991500.024.8554320-21
AGAGTAC10092000.024.79229218-19
TACATGG10104050.024.51763222-23
CATGGGG6342850.024.25355124-25
GTACATG9654600.022.70328922-23
CGCAGAG10021400.022.15108316-17
TCAACGC10052050.022.02365312-13
Produced by FastQC (version 0.11.5)