FastQCFastQC Report
Wed 13 Jun 2018
HW2TVBGX5_n01_otdbcdhd-gp6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TVBGX5_n01_otdbcdhd-gp6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11382
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571850.237216657880865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG450.3953610964681075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG390.3426462836056932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT390.3426462836056932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG300.2635740643120717No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGG250.2196450535933931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGGGGGGG180.158144438587243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG180.158144438587243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG170.1493586364435073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG140.12300123001230012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG140.12300123001230012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG140.12300123001230012No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGG140.12300123001230012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGCC150.002168425170.057
CTGCCTG150.002168425170.059
GTTGCTG150.002168425170.059
TTGCTGG150.002168425170.060
GTGTTCG200.00677689152.557
TGCTGGG200.00677689152.561
TGCCTGG305.635506E-446.66666460
GTGGGTT305.635506E-446.66666445
TGGGTTT305.635506E-446.66666446
CCTGGGG305.635506E-446.66666462
CGTGGGG350.001203981740.062
TGGGTGT350.001203981740.028
GCCTGGG400.002320245435.061
TGCGGGG400.002320245435.061
CTGGGGG1001.0444637E-831.49999863
GTGGGTG604.6838098E-429.16666627
GGTGGGT604.6838098E-429.16666626
GCTGGGG800.00250945621.87562
GGGGTTG900.00496792519.44444523
TGTGGGG1200.001233551517.532
Produced by FastQC (version 0.11.2)