FastQCFastQC Report
Wed 13 Jun 2018
HW2TVBGX5_n01_otdbcdhd-gp5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TVBGX5_n01_otdbcdhd-gp5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550543.69047619047619No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG860.6825396825396826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG690.5476190476190477No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG590.4682539682539682No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG380.3015873015873016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT370.29365079365079366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG360.2857142857142857No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGG320.25396825396825395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG310.24603174603174605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG260.20634920634920637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGG260.20634920634920637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGGGGGGG220.1746031746031746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG190.1507936507936508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGTGGGGGGGGGGGGGGGGGG150.11904761904761905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGT150.11904761904761905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG130.10317460317460318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.002174045270.014
CACACGT150.002174045270.013
ACGTCTG150.002174045270.016
CACGTCT150.002174045270.015
AAGAGCA150.002174045270.08
GATCGGA150.002174045270.02
GTCTGAA150.002174045270.018
GAAGAGC150.002174045270.07
TCGGAAG150.002174045270.04
AACTCCA150.002174045270.023
GAGCACA150.002174045270.010
AGAGCAC150.002174045270.09
GAACTCC150.002174045270.022
ATCGGAA150.002174045270.03
GGAAGAG150.002174045270.06
AGCACAC150.002174045270.011
TGAACTC150.002174045270.021
AGATCGG150.002174045270.01
GCACACG150.002174045270.012
CTTCTGC150.002174045270.055
Produced by FastQC (version 0.11.2)