FastQCFastQC Report
Wed 13 Jun 2018
HW2TVBGX5_n01_otd-gp6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TVBGX5_n01_otd-gp6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9387
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG426745.456482369234045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG890.9481197400660488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT880.9374667092787898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG750.7989773090444231No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGG540.5752636625119847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG440.4687333546393949No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG370.39416213912858206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG360.38350910834132307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG330.3515500159795462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGG300.31959092361776925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG260.27697880046873335No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG190.20240758495792052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGTGGGGGGGGGGGGGGGGGG160.1704484925961436No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGGGGGGG120.1278363694471077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG110.11718333865984873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGT110.11718333865984873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTGT207.5252094E-570.028
GGTGTTG307.750985E-658.33333223
GGGTGTT252.2700889E-455.99999622
GCGGGCG200.006739397552.541
GGTTGGC200.006739397552.543
CGGGCGG200.006739397552.542
CGTGGGG400.002299181435.062
GTGGTGG400.002299181435.054
CCGGGGG500.006856207327.99999863
GGGGTGT657.3814456E-426.92307721
TGTGGGG750.001701221523.33333432
CGGGGGG2951.2732926E-1117.7966164
GCGGGGG2657.4212367E-613.20754763
GGCGGGG2751.6130567E-411.45454562
GTGGGGG3551.1777145E-510.84507133
GGGCGGG2400.00953414310.20833361
TGGGGGG3903.3387223E-59.87179534