FastQCFastQC Report
Wed 13 Jun 2018
HW2TVBGX5_n01_otd-gp5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TVBGX5_n01_otd-gp5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36776
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2676172.76756580378508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG2790.7586469436589081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG2200.5982162279747661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG1910.5193604524690015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG1400.38068305416576026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGG1330.36164890145747225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG1290.35077224276702196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG1140.30998477267783336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT1100.2991081139873831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG770.20937567979116817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGGGG750.20393735044594302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGG730.19849902110071785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG700.19034152708288013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGG560.1522732216663041No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGG450.12236241026756581No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGG420.11420491624972809No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGGGGGGGGGGG370.10060909288666521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGG305.805019E-446.66666850
TGGGTGT350.001239976740.046
GGTGTGT400.002389189835.049
GGGGTGT1003.8346366E-421.00000247
GGGTGTG1055.3295755E-420.048
GGTTGGG1000.009518255517.50000243
TGGGGTG1250.001719178316.846
GGGGTTG1450.00462515914.482758523
GGGGGTT1707.912853E-414.41176540
CGGGGGG5905.797847E-89.49152662
GGTGGGG10250.08.87804932
GTGGGGG10650.08.873239533
GGGTGGG10151.6370905E-118.27586231
GCGGGGG5502.2115697E-58.27272761
TGGGGGG10705.456968E-128.1775734
GGGGGTG10807.2759576E-128.10185129
GGGGTGG10756.184564E-117.81395330
GGCGGGG4550.00211832377.692307560
GGGGGGT12251.4006218E-107.142856628