FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25407705
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2478820.9756174357345538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC622250.035.32011455
CGCCGTA689350.034.82129753
TCGCCGT671750.034.81780652
CGTATCA660050.034.51892556
GTCGCCG811450.034.26036551
GCCGTAT776700.033.9468654
CTCGGTG664150.033.89023644
TCTCGGT651700.033.81627743
GATCTCG641900.033.78165441
GGTCGCC847300.033.59093550
CGAGATC141350.033.3818245
TGGTCGC778250.033.2841649
ATCTCGG703850.033.187542
AGATCTC678950.032.30074740
GTGGTCG855250.031.4878448
GTATCAT638700.031.32685957
TCGGTGG863700.031.07898745
GGTGGTC868200.031.00894547
CGGTGGT905200.030.4856446
TAGATCT789000.030.38190339
Produced by FastQC (version 0.11.5)