FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5820135
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92120.15827811554199345No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG69290.1190522212972723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC121550.033.34355555
CGCCGTA134800.032.92210853
TCGCCGT135150.032.6814752
GTCGCCG153900.032.33845551
CGTATCA127550.032.18665356
GCCGTAT151250.031.60920754
GGTCGCC163000.031.52076750
TGGTCGC155350.031.22556149
CTCGGTG130100.031.1522644
GATCTCG126400.030.81813641
TCTCGGT130850.030.78646943
ATCTCGG136500.030.64033942
GTGGTCG163300.030.21977648
GTATCAT120800.029.40745257
GGTGGTC166300.029.3589347
AGATCTC138400.028.75296840
TCGGTGG163900.028.46488845
TAGATCT157050.027.5900339
CGGTGGT179750.027.08423246
TCATTAA156250.026.43140660
Produced by FastQC (version 0.11.5)