FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231044
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144840.1759679574061322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC226700.028.11353955
CGCCGTA253150.027.52646353
CGTATCA235750.027.44999756
TCGCCGT252450.027.28392252
GTCGCCG292650.026.63353351
GCCGTAT280200.026.63030454
GGTCGCC307000.026.3348450
CTCGGTG248100.026.28107844
GTATCAT218000.026.24933657
TCTCGGT248050.025.87719243
ATCTCGG260850.025.8151142
CGAGATC42600.025.7977855
GATCTCG245350.025.54872341
TGGTCGC293550.025.52652249
TCATTAA257200.025.33761860
CATTAAA276250.024.90796961
GTGGTCG308800.024.70792448
GGTGGTC314300.024.56508347
AGATCTC257550.024.48797840
ATCATTA262750.024.37616759
Produced by FastQC (version 0.11.5)