FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399527
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164960.17549819262182023No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC225500.031.0570255
CGCCGTA252050.030.2572753
TCGCCGT252900.029.7265652
CGTATCA238450.029.54647656
GCCGTAT279400.029.4500154
GTCGCCG289500.029.34137351
GGTCGCC302450.028.80252550
TGGTCGC291800.028.2944949
GTATCAT227550.027.94713857
CTCGGTG252600.027.8220844
ATCTCGG265100.027.38156542
GTGGTCG310200.027.24799548
GATCTCG248850.027.228741
TCTCGGT256500.027.2216743
GGTGGTC314000.026.79563547
CGAGATC46400.026.6268985
TCATTAA276800.025.79427560
AGATCTC268200.025.6949940
TCGGTGG320000.025.68072945
CATTAAA294500.025.6463461
Produced by FastQC (version 0.11.5)