FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4137441
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78990.19091510912179774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC90200.032.67074655
CGCCGTA98100.032.35875353
TCGCCGT97450.031.8922152
CGTATCA97250.031.6698956
GCCGTAT114100.031.34864254
GTCGCCG131200.031.34422551
GGTCGCC132150.031.0659350
AAAGGGG67300.030.3195570
TGGTCGC117250.029.93929749
ATCTCGG124750.029.71042842
GTATCAT94550.029.6129757
GATCTCG122100.029.35200741
CTCGGTG118150.029.29652244
TCTCGGT115550.028.83503543
CCGGATC32950.028.57264155
TCGCCGG35100.028.51756352
GGGTCGC23750.028.44115349
CATTAAA125800.028.1826861
CGAGATC24500.028.1429355
GTGGTCG133950.028.00951448
Produced by FastQC (version 0.11.5)