FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066566
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69780.1715944115993691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC122200.031.73408555
CGTATCA127900.031.1681256
CGCCGTA136950.031.10183153
TCGCCGT136550.030.7059552
GTCGCCG160900.030.53996551
GCCGTAT151550.030.2994954
CTCGGTG134300.030.2300944
GTATCAT117800.030.21573357
GGTCGCC167850.030.02607550
GATCTCG132750.029.9239441
TCTCGGT134900.029.68052343
TGGTCGC157400.029.52913949
ATCTCGG141700.029.49116142
TCATTAA145350.028.55795360
AGATCTC141000.028.5205840
GTGGTCG168000.028.45763648
CATTAAA155950.028.36737461
GGTGGTC169200.028.13170247
ATCATTA144750.027.99931759
TCGGTGG171450.027.57879445
Produced by FastQC (version 0.11.5)