FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996151
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89880.22491642583075563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG144900.038.45439570
CGAGATC31900.036.6455845
TCGCGAG2350.032.7731742
CCGTATC237100.031.67766455
CGTATCA243800.031.36698356
CATTAAA262750.031.12941761
CTAGATC29350.031.0049555
GCGAGAT12900.030.9300794
CGCGCAG1700.030.9019181
GTATCAT223050.030.7074457
CGCCGTA269900.030.62894453
TCGCCGT267700.030.61917952
TCATTAA252250.030.57985560
GTCGCCG311250.029.93323751
GATCTCG244500.029.70258541
CGCGAAA5900.029.679811
CGCGTAG2600.029.6219082
GCCGTAT294450.029.5847854
GGTCGCC327700.029.54137650
CAAGATC50500.029.5246035
Produced by FastQC (version 0.11.5)