FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3691059
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81930.22196881707932603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC26450.037.712185
AAAGGGG93050.037.1247370
CCGTATC136250.035.0637755
CGTATCA145500.033.9170956
CGCCGTA144700.033.5966753
CTAGATC26000.033.5188035
TCGCCGT138500.033.45805752
GTCGCCG190300.033.27072551
CAAGATC41400.033.139675
GGTCGCC193100.032.96954350
TGGTCGC165400.032.777749
GCCGTAT173350.032.60702554
TCGCGTA2900.032.603461
ATCTCGG185500.032.22600642
GATCTCG177900.032.14686241
AGATCTC171300.032.09824840
TCGCCGG51650.031.71305352
CTCGGTG175550.031.56040644
CCGGATC49850.031.3837555
TCTCGGT169950.031.36470843