FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579963
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107680.23511106967458034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC40450.041.1863825
AAAGGGG131200.037.9083570
CTAGATC39700.036.145865
CCGTATC221700.034.35175355
CGCCGTA243750.034.0154453
TCGCCGT235950.033.73076652
CAAGATC59650.033.6795775
CGTATCA237350.033.4875756
GTCGCCG313150.033.05989551
GGTCGCC317600.032.9713650
GCCGTAT283400.032.8625154
GATCTCG279150.032.52280841
AGATCTC272400.032.40363340
CATTAAA288400.032.32917861
ATCTCGG292200.032.3279742
TGGTCGC277800.032.302949
CGCGAGA7000.032.0022353
TCATTAA277550.031.99152460
GGGTCGC51750.031.9894349
CCGGATC73050.031.52545755
Produced by FastQC (version 0.11.5)