FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807189
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50120.1785415944562336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC16250.037.046395
CCGTATC106600.035.55775555
AAAAGGG78700.035.26643470
CGTATCA111150.035.17278756
TCGCCGT118300.034.76291752
CGCCGTA120950.034.6957653
CTCGGTG110200.034.07855644
GATCTCG104450.033.97759241
GTCGCCG136100.033.7137951
TCTCGGT108400.033.67581643
ATCTCGG112200.033.53348542
GCCGTAT133700.033.50747354
CGCGCAG1055.493348E-1033.3507731
AGATCTC107650.033.1301440
GGTCGCC143700.032.83191350
TGGTCGC136750.032.60657549
TCGGTGG138000.032.3873145
CAAGATC23700.032.341985
GTGGTCG141850.032.05109848
GGTGGTC144600.031.68359447
Produced by FastQC (version 0.11.5)