FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938271
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55180.18779751765579145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC15050.030.9297755
AAAAGGG82100.030.39644270
CCGTATC102600.030.12138655
CGTATCA105100.030.03761356
TCGCCGT114400.029.92088552
CGCCGTA114900.029.85160453
GTCGCCG137300.029.2384651
GCCGTAT128150.028.81346554
TGGTCGC133250.028.78756749
GGTCGCC144950.028.7819150
CTCGGTG115700.028.7679444
AAAGGGG31900.028.52719370
TCTCGGT113450.028.44382543
GATCTCG112850.028.03680241
ATCTCGG122150.028.02250542
GTGGTCG141250.027.87569248
AGATCTC116800.027.5381240
GGTGGTC141500.027.52962747
GTATCAT102700.027.46794757
TCATTAA120100.026.8688860
Produced by FastQC (version 0.11.5)