FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512720
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133620.24238488441277625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC30000.036.0506485
CCGTATC206900.032.52961355
CGCCGTA227450.032.22187453
TCGCCGT224150.032.13414452
AAAGGGG77300.032.05633570
GTCGCCG272700.032.0346251
GGTCGCC279700.031.77096250
CGTATCA217250.031.60816856
AAAAGGG189050.031.34298970
TGGTCGC257150.031.31772449
GCCGTAT254750.031.31051854
GATCTCG228950.031.30757341
ATCTCGG241150.030.92831642
CTCGGTG232050.030.75358844
AGATCTC232550.030.67241540
TCTCGGT228450.030.54879643
CCGGATC46150.030.03195455
GTGGTCG278850.029.92235248
GGTGGTC279450.029.8706347
CATTAAA257050.029.73686261
Produced by FastQC (version 0.11.5)