FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.17768301350390903No Hit
CACAAAAGGAAATACGAAAAGTGAGGGATCAACTGAAAGAGGAAACTCTG30.10660980810234541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA200.006253135452.50000460
GATCGGA351.5977803E-550.08
ATCGGAA351.5977803E-550.09
AGATCGG351.5977803E-550.07
CGGAAGA304.9265724E-446.66666411
GAGATCG304.9265724E-446.6666646
TCGGAAG403.5214434E-543.75000410
GGAAGAG350.001053351140.012
GCGTCGT350.001053351140.018
CGTCGTG350.001053351140.019
GTCGTGT400.002031550335.00000420
TCGTGTA400.002031550335.00000421
AGGGAAA400.002031550335.00000427
GGAAAGA450.00362147531.11111329
GAAAGAG500.00606692628.00000230
Produced by FastQC (version 0.11.5)