FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6198971
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126050.20334019952666338No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG91010.14681468908307524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC122000.034.88474755
TCGCCGT131950.034.77403652
CGCCGTA134200.034.53005253
GTCGCCG150500.034.48787751
CGTATCA128300.033.7446456
GGTCGCC158200.033.4729750
TGGTCGC151750.033.2120449
GCCGTAT149000.033.14381454
CTCGGTG124350.032.73374644
ATCTCGG130200.032.17695642
TCTCGGT125500.031.93181243
GATCTCG122000.031.90118241
GTGGTCG161450.031.86699348
GGTGGTC165350.031.13653447
GTATCAT125150.030.65077657
TCGGTGG160400.030.11178445
AGATCTC133450.029.89840940
CGAGATC24650.028.681135
TAGATCT152500.028.43588439
CGGTGGT177300.028.36676446
Produced by FastQC (version 0.11.5)