FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281177
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG104520.2441384693975512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC47900.033.97620850
ATCTCGG46650.033.91128542
GTCGCCG48500.033.77237351
CCGGATC20100.033.4324155
TCGCCGG20250.033.0115452
CCGTATC27850.032.92597255
CGGATCA22350.032.8854956
GATCTCG46400.032.81170341
CTCGGTG46900.032.68577244
GCCGTAT41050.032.39910554
TCTCGGT42800.032.30061343
TGGTCGC36600.032.2259949
CGCCGGA22350.031.94590453
TCGCCGT29850.031.7747752
CGCCGTA31200.031.74639153
GCCGGAT30600.031.45385254
AGATCTC44450.029.9996240
GGGTCGC21750.029.93030549
GTGGTCG52750.029.79074348
GTGGCCG31200.029.7268451
Produced by FastQC (version 0.11.5)