FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n02_ncm5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n02_ncm5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691277
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98850.17368685446166124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC139350.034.35878855
CGCCGTA154150.033.557553
TCGCCGT149650.033.42059352
CGTATCA149000.033.14357856
GTCGCCG177850.032.9624651
GCCGTAT172700.032.648454
GGTCGCC184050.032.51763550
CTCGGTG151350.032.1433444
TGGTCGC170250.032.04923249
ATCTCGG161050.031.8155242
TCTCGGT152950.031.57826643
GATCTCG152000.031.52234641
GTGGTCG182850.030.68295948
GTATCAT140200.030.60561457
GGTGGTC186600.030.10385147
AGATCTC160350.030.01182440
CGAGATC26200.029.9230185
TCGGTGG193900.029.6022545
CGGTGGT204500.028.59840446
AAAGGGG58550.028.39442370
Produced by FastQC (version 0.11.5)