FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25407705
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1532400.6031241310460744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT168650.038.6545141
AAGATCG882350.029.147116
GAGATCG616650.029.0653486
TGAGATC211700.026.0120035
GTCACAG1035450.025.94440336
AAAGATC323850.025.7004385
TCACAGT1030850.025.6586337
CACAGTC646950.025.4796438
AGTCACA1343600.025.39120335
CAGTCAC2062850.025.196734
GCGAGAT95550.025.1545184
CCAGTCA2068850.025.09352933
TCCAGTC2110100.025.07047732
ACGTCTG2193050.024.97654222
CGTCTGA2181000.024.96923323
GAACTCC2098700.024.94591928
CACACGT2211650.024.90950619
ACACGTC2225500.024.81315620
TGAACTC2122900.024.81038327
CTGAACT2140200.024.75178126
Produced by FastQC (version 0.11.5)