FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5820135
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA41750.030.34699866
GGCGTGA17750.029.7743565
GCCGTCT146050.029.35608956
ACACTCT33850.029.06361
TGCCGTC193950.029.01749655
TATGCCG219450.028.56430653
GCTTGAA96600.028.40551266
GCCGTCG47100.028.38612656
ATGCCGT215050.028.1885154
CCGTCTT154000.027.8406357
TCGGCGT18750.027.81305363
GTATGCC228200.027.7911352
CGGCGTG19000.027.26288464
CCGTCGT49450.027.24947457
GATCTCG203050.027.04479246
CTGCTTG79650.026.93632764
TCTCGTA209700.026.63778548
TGCGTGA29050.026.62623665
ATCTCGT202650.026.58004247
CTGCGTG28950.026.47641864
Produced by FastQC (version 0.11.5)