FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231044
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT83400.043.3993531
CCGGGGT13000.023.42293557
CACTCTT174550.022.5812151
TGGCGGG58200.022.5514155
TCGGGGA24850.022.53507648
TGCCGGG20500.022.0242655
CGGGGAT19450.021.95359849
GGGTGGA28800.021.8748766
ATCGCGG43150.021.73799147
CGGGGTT66300.021.37996349
TCGGGGT84350.021.24468648
GCGAGAT29050.021.0853634
GAGATCG205250.020.7196466
CCGCGAG4450.020.4504362
AATCGCG56000.020.37487846
GGGGGAA67550.020.36257267
ATCGGGG278750.020.31557347
AATCGGG315950.020.10590746
CGCGAGA11500.019.7835713
GGGGAAA75750.019.77545768
Produced by FastQC (version 0.11.5)