FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399527
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT61100.034.3797071
GGGTGGA34400.024.7236766
ATCGGGG146200.024.05934147
CATCGGG178150.023.83087746
TGCCGGG24950.023.28641355
CCGGGGT16600.022.98177157
GAGATCG192600.022.2436666
TCGGGGT59700.022.1606548
GATCATC331600.022.15455443
TGGCGGG68750.022.14530255
CATCGCG55900.021.66353646
ATCGCGG40300.021.62516247
AGATCAT478600.021.55125242
CAGATCA531750.021.37173841
GCGGGGT61150.021.34899557
GGGGGAA76800.021.3279867
CGGGGTG67950.021.32435658
CGGGGTT52550.021.3128949
TTGCGGG40150.021.00857455
TCATCGG215250.020.95920645
Produced by FastQC (version 0.11.5)