FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4137441
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT28900.039.24621
GGGCGTG15250.032.3605464
GGGTGGA19050.031.60093566
GCGTGAA28050.031.44373766
GCGTGGA15850.030.91471566
GTCGGCG11400.030.39462762
GGCGTGA16250.029.93835465
GCCGTCG28800.029.40961556
TATGCCG99600.029.1314253
TGGGCGT16950.028.08249563
TCGGCGT18200.028.0768263
ATGCCGT81600.028.00847654
CCGTCGT31650.027.75661557
CGTGAAA35100.027.720767
TGCCGTC69350.027.65671355
GTATGCC113400.027.40730952
CCGGCGT19100.027.30356857
GCCGTGG17050.027.30195456
CCGGGGT15950.027.20993257
GCCGGCG20000.027.12490356
Produced by FastQC (version 0.11.5)