FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066566
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT26650.040.9848671
GGGTGGA22400.030.46851566
CCCGGGT5400.027.22201257
CGGGGTG38600.027.02051758
GGGGAAA43250.026.38129868
GGGGGAA37300.026.36708867
CCGGCGT9450.026.29609157
CCGGGGT14200.026.12655857
GCCCGGG5500.026.09070656
TGCCGGG18950.026.04201555
GCGGGGT32050.025.88123757
GATCGCG13350.025.69268646
CCGGGTG10450.025.11942358
GGAAAAA95950.024.7314470
GAGATCG103350.024.7231466
ATCGCGG8700.024.54004147
GGGGGGA41600.024.39884866
GGTGGAA29150.024.3737467
TTGGCGG22300.024.17021654
GTGGGCG13350.023.85749462
Produced by FastQC (version 0.11.5)