FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996151
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT53400.053.6246221
GCGTGAA47550.037.3919866
GGCGTGA22500.036.39976565
CGTGAAA52150.035.97292367
GCGTGGA22400.035.93726766
GAAAAAA224200.034.32850370
TCGGCGT24500.034.28549663
GGTCGGC7600.033.61820661
GGGCGTG23500.033.2125564
GCTTGAA57600.032.38694466
GGGTGGA26600.031.57874366
GTGAAAA89650.031.38853368
GTGGAAA54100.031.31218368
TGGGCGT24450.031.2063463
TGCGTGA29000.031.01704265
CTGCGTG26100.030.97681264
CGTGGAA27150.030.93902867
GTCGGCG18450.030.92121162
GCCGTGT25200.030.55535956
CGTGGGC15800.029.68335361
Produced by FastQC (version 0.11.5)