FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3691059
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT41350.055.0275841
GGAAAAA102450.034.88027670
GGGTGGA29500.033.93205366
GCGTGGA6650.033.15774566
AAAGATC58100.032.4706155
CCGGGTG23900.031.92454358
GTGGAAA42950.030.64014268
TGAGATC41050.030.6097055
GAGATCG144950.030.5698536
GGAGATC25550.030.2746585
GCGAGAT18300.030.0280024
GCCCGGG9450.029.62949856
GGTGGAA35500.029.57733367
CACTCTT91350.029.008811
CCGCGTG6650.028.94723958
TGCCCGG8750.028.79987155
GTCCGAG2800.028.750652
GGATGCC10800.028.5183952
GTGAGAT17200.028.2855264
CCCGGGT10400.028.26910657
Produced by FastQC (version 0.11.5)