FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579963
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT58100.053.7475741
GGAAAAA155800.036.0781270
GGGTGGA48150.035.2542366
GCGTGGA11900.033.23512366
AAAGATC82000.032.396885
GTGGAAA66050.032.37682368
GGTGGAA54250.031.93531867
GGGTGTA23500.031.87217566
CCGGGGT28000.031.62483857
GTGAGAT22550.031.3530734
GGAGATC36850.030.6789385
GAAAAAA147250.030.63821470
ATGCCGG19550.030.61365354
TGAGATC62300.030.562315
GAGATCG213950.030.4281776
GCGAGAT24650.030.3859044
TGGGCGT14450.030.27665963
CACTCTT134100.030.2047141
TGCCGGG40250.029.65202155
TGCCCGG11050.029.45686155
Produced by FastQC (version 0.11.5)