FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807189
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT14550.045.7108761
GCGTGAA35550.035.147666
CGTGAAA38500.034.2726567
GTGCGTG7050.033.26233764
GGCGTGA16650.033.00292665
GCTTGAA48250.032.93256866
GCGTGCA3300.032.8787166
TCGGCGT17300.032.5721863
CTGCTTG38450.032.3146264
TGCCGGG8350.032.2753855
GAGATCG69650.031.9597266
GAAAAAA143300.031.70264870
GCCGTCT88250.031.52967356
GCCGTGT13800.031.44920256
TGCGTGA21800.031.30726865
CCGTCTT89550.031.26737857
AAGATCG102800.031.0164666
TATGCCG159500.030.94036953
CTGCGTG20300.030.86264
CCGTCGT39500.030.65815757
Produced by FastQC (version 0.11.5)