FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938271
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT27250.046.1162261
GGCGTGA14850.038.18169867
GCGTGAA30050.037.96993368
CGTGAAA33200.036.26494669
GTGAAAA51300.033.70359870
CTGCGTG16500.033.30292566
GGGGTGA9400.033.13819567
TGCGTGA18450.031.86981867
GGGCGTG10400.031.63451666
TCGGCGT16100.030.21729565
CCGTCGT36650.030.1772659
TCGTCGG13750.030.03626862
GTCGGCG11550.029.99990564
GCTTGAA38400.029.34886668
CACTCTT65400.029.3311181
TGGGCGT10700.029.11205765
GCCGTCG40150.028.4183458
CGGCGTG18650.028.33771366
GCGTGGA7850.028.0890868
CGTCGGC13300.027.8946563
Produced by FastQC (version 0.11.5)