FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5512720
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT51100.047.8867381
GGGGAAA48550.032.87305570
GGGTAAA32200.027.60846770
GAGATCG161650.026.893166
CGGAGTC7950.026.8551247
CACTCTT115000.026.7577781
TGAGATC52450.026.4935995
AAGATCG230300.026.0807046
GCGAGAT21200.025.7563724
ACGAGTG7750.025.74172438
AAAGATC71450.025.7189395
GGGAAAA36600.025.43694770
GGGGGAA58200.025.25752369
TTGCGGG24350.025.15379557
AATCGCG58550.025.0467648
ATCGCGG51350.025.01439949
CGGGGGT103450.024.96837651
GGAGATC30250.024.6462125
AATCGGG260300.024.1219748
TCGCGGG35300.023.99413550
Produced by FastQC (version 0.11.5)