FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAACGGAGTCACTCAGCTTGATGCTATTTAACCCACGAGTGAGGTCT30.10660980810234541No Hit
ATTTAGGAATAACTTTAATATTACCTTTTCCAGATATATTTTTAATATAC30.10660980810234541No Hit
ATCGTTTGCTTTCGGGTCAATTAATATTAATTAAATATTTTAATATATTT30.10660980810234541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG200.006253135452.50000447
ATCTCGT200.006253135452.50000448
CCGTCTT200.006253135452.50000458
TCTCGTA200.006253135452.50000449
AAGATCG200.006253135452.5000046
TAATCTC200.006253135452.50000446
GTCTTCT200.006253135452.50000460
GCCGTCT200.006253135452.50000457
TCTTCTG200.006253135452.50000461
CGTCTTC200.006253135452.50000459
CTCGTAT200.006253135452.50000450
CTTCTGC200.006253135452.50000462
ACGTCTG351.5977803E-550.022
CACGTCT351.5977803E-550.021
CTCCAGT351.5977803E-550.031
CGTCTGA351.5977803E-550.023
GTATGCC304.9265724E-446.66666453
CAGTCAC304.9265724E-446.66666434
CACACGT304.9265724E-446.66666419
CCAGTCA304.9265724E-446.66666433
Produced by FastQC (version 0.11.5)