FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6198971
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGG21050.027.9333955
GAGATCG86800.027.6213156
CCGGGGT17500.027.399957
AATCGCG25900.026.89179646
AAGATCG127950.026.670916
AGTCACC280900.026.4274635
CCTTGTA275250.026.42315540
CGGGGTG37200.026.24990558
ACCTTGT275500.026.19591539
ATCGCGG14700.026.19038247
CAGTCAC284800.026.1761734
ACACTCT30750.026.0693651
CCAGTCA286600.026.03619633
TCCAGTC293950.025.77810132
GGGTGGA26750.025.64476866
GTCACCT289950.025.56638536
GTAATCG45000.025.5110244
CACCTTG287400.025.50095438
CGTCTGA298600.025.49388123
GCGGGGT28500.025.42096157
Produced by FastQC (version 0.11.5)