FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281177
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA25950.033.3138366
TGGGCGT13950.031.36178863
TGCCGGG16750.030.71620255
GCCGTCT40950.030.42713756
GGCGTGA14450.030.27660265
TATGCCG94100.029.97853753
TCGGCGT14700.029.76169663
CATCTCG130450.029.53984646
CCGGGGT13900.029.46022657
CGTGAAA32000.028.9841767
TGCCGTC67800.028.65024455
GTATGCC103050.028.6315352
ATCTCGT123950.028.5758447
ATGCCGT80750.028.56326754
GCGTGGA13500.028.51831866
GGGCGTG14700.028.33313664
GAGATCG82950.027.638236
GGCTACA224700.027.6166341
GCTACAT210000.027.54980742
CTCGTAT104500.027.53090949
Produced by FastQC (version 0.11.5)