FastQCFastQC Report
Thu 31 Oct 2019
HW2TNAFXY_n01_ncm5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2TNAFXY_n01_ncm5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691277
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT41000.037.3994261
TGGCGGG39300.029.7452755
TGCCGGG16850.027.00271655
ATCGGGG161450.026.75106847
TCGGGGT57600.026.7358648
TATCGGG195850.026.39495546
CGGGGTT48000.026.32267449
GAGATCG117100.025.676436
GCTTATC299950.025.56569143
CTTATCG255000.025.50172444
AAGATCG165450.025.3659296
TTATCGG211000.025.34573645
CCGGGGT11500.025.26063357
TAGCTTA354550.025.14290641
AGCTTAT335550.025.09587742
ACTAGCT364150.024.87413439
CTAGCTT366000.024.65277540
CACTAGC372750.024.48803738
TCACTAG376550.024.29668237
GCCCGGG6800.024.19095256
Produced by FastQC (version 0.11.5)