FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-zmarr2u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-zmarr2u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12843142
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT559770.43585128934959994TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA254340.19803565202346904No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT201070.1565582627677869No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA178510.1389924677310272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC80300.055.13611246
TCGTATG80900.054.8137245
CTCGTAT80700.053.34488344
TATGCCG83900.052.85313448
AGTCACG88050.050.5641428
GCCTTAT86700.049.8969637
TATCTCG85250.049.5534741
GTATGCC90250.049.251347
TCACGTG97700.045.56929830
TTATCTC93450.045.39236540
GGCCTTA97900.044.90347736
TCTCGTA94650.044.78006443
CTTATCT94750.044.6596239
CCTTATC95900.044.12442438
ACGTCTG104300.043.86041615
CAGTCAC103650.043.05537827
GTCACGT105750.042.0678229
CACGTGG106200.041.88924431
CACGTCT110550.041.5703914
GTCTGAA112200.040.73942617
Produced by FastQC (version 0.11.2)