FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-zmarr2u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-zmarr2u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6498747
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC252050.3878439951578358TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA137870.21214858802781522No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA69520.10697446753966573No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT66980.10306602180389544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36800.053.8305646
TCGTATG36800.053.73710343
CTCGTAT34750.053.2812642
CGTATGC37650.053.17465244
GTATGCC40850.048.92276445
CAGTCAC46600.043.7901427
AGTCACC46600.043.71502328
ACGTCTG47800.043.12717415
CACGTCT50300.040.84451314
GTCTGAA51300.040.45736317
TCTCGTA44300.040.45198441
CCAGTCA54100.037.84910626
CGTCTGA55000.037.48143416
CCGTCTT54300.036.80384450
AAAAGGG51850.035.97758570
AGGCGAT54000.035.7790635
TCTGAAC58400.035.47854218
TGAAAAA57300.035.42775762
AGCACAC60400.034.8263410
CACACGT60250.034.68041212
Produced by FastQC (version 0.11.2)