FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-zmarr2s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-zmarr2s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10476400
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT681150.6501756328509793TruSeq Adapter, Index 27 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA331150.3160914054446184No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT173610.16571532205719522No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA160210.15292466877935168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC88400.058.83448446
CTCGTAT87250.058.56670444
TATGCCG89800.057.64386748
TCGTATG90600.057.36692445
GTATGCC92250.056.3411247
TATCTCG91750.055.0075841
TCTCGTA99100.050.99843643
TTATCTC99750.050.5260140
GTCACAT105000.050.2349529
ACATTCC104100.050.1286632
AGTCACA105300.049.9588728
CCTTTAT103450.049.70027537
CTTTATC103300.048.78963538
CAGTCAC109450.048.0965627
ACGTCTG113900.046.95385715
CACATTC112400.046.58468231
TCACATT114350.046.03536230
TCCTTTA113750.045.93818336
CACGTCT117350.045.6627114
CCGTCTT114550.045.3419452
Produced by FastQC (version 0.11.2)