FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-zmarr2s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-zmarr2s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9908264
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC475890.4802960437872871TruSeq Adapter, Index 3 (97% over 37bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA150180.1515704466493828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC65050.054.1809544
TCGTATG65200.054.10997843
TATGCCG65800.053.5104646
CTCGTAT65200.053.0903242
TATCTCG68100.050.00642439
GTATGCC70600.049.87210545
ACCTAGC72800.048.70250732
CACCTAG73900.048.0244431
TCTCGTA76600.044.68608541
AGCTATC77000.044.63644436
AGTCACC79900.044.32959728
TAGCTAT78700.044.25061835
GCTATCT77500.044.16782437
CCTAGCT82000.043.32368533
ACGTCTG83100.043.21394715
CAGTCAC83150.042.5548427
CTATCTC80900.042.3113638
CACGTCT86950.041.58207314
CGTCTGA89450.040.06714616
AAAAGGG83550.039.6296470
Produced by FastQC (version 0.11.2)