FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-6597u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-6597u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17852474
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1207530.6763936471774163TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA373730.20934353412305767No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA293000.16412291091979747No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT292970.1641061065261879No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG207790.11639283160417992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC159300.059.43084346
TATGCCG159400.059.08582348
TCGTATG159950.058.9267545
CTCGTAT158950.057.8442144
AGTCACG169650.056.40636428
GTATGCC169450.056.05699547
ACGTCCG180900.052.8385432
TCTCGTA178250.050.97245443
TCCGCAC187800.050.74823435
GTCACGT188250.050.7401829
CAGTCAC192150.049.96562627
ACGTCTG195200.049.7036615
CGCACAT190600.048.8457137
CCGCACA194200.048.6611336
CACGTCT200050.048.4635214
GTCCGCA198250.048.16177734
GTCTGAA206600.047.095417
TCACGTC205050.046.65112730
CCGTCTT201900.046.57851452
ATGCCGT205850.045.97376649
Produced by FastQC (version 0.11.2)