Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-6597u2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8228061 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC | 32773 | 0.3983076936352319 | TruSeq Adapter, Index 7 (97% over 35bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 19792 | 0.2405426989420715 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 16225 | 0.19719105145185484 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 10728 | 0.1303830878259167 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 4915 | 0.0 | 55.75719 | 44 |
TCGTATG | 5000 | 0.0 | 54.66866 | 43 |
TATGCCG | 5065 | 0.0 | 53.691338 | 46 |
CTCGTAT | 5005 | 0.0 | 52.236797 | 42 |
GTATGCC | 5355 | 0.0 | 51.241196 | 45 |
ACTCAAT | 5785 | 0.0 | 46.76834 | 35 |
ACGTCTG | 6160 | 0.0 | 46.195133 | 15 |
AGTCACC | 6250 | 0.0 | 44.297333 | 28 |
CACGTCT | 6425 | 0.0 | 44.28981 | 14 |
CCACTCA | 6250 | 0.0 | 44.072525 | 33 |
CGTCTGA | 6630 | 0.0 | 42.865753 | 16 |
TCTCGTA | 6065 | 0.0 | 42.818924 | 41 |
CAGTCAC | 6525 | 0.0 | 42.645218 | 27 |
GTCTGAA | 6745 | 0.0 | 42.498135 | 17 |
ACCACTC | 6665 | 0.0 | 41.485107 | 32 |
CACTCAA | 6675 | 0.0 | 41.16154 | 34 |
CTCAATC | 6305 | 0.0 | 40.967724 | 36 |
CACACGT | 7065 | 0.0 | 40.723347 | 12 |
AATCTCG | 6370 | 0.0 | 40.549194 | 39 |
AAAAGGG | 6490 | 0.0 | 38.666233 | 70 |