FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-6597u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-6597u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228061
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC327730.3983076936352319TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA197920.2405426989420715No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA162250.19719105145185484No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT107280.1303830878259167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49150.055.7571944
TCGTATG50000.054.6686643
TATGCCG50650.053.69133846
CTCGTAT50050.052.23679742
GTATGCC53550.051.24119645
ACTCAAT57850.046.7683435
ACGTCTG61600.046.19513315
AGTCACC62500.044.29733328
CACGTCT64250.044.2898114
CCACTCA62500.044.07252533
CGTCTGA66300.042.86575316
TCTCGTA60650.042.81892441
CAGTCAC65250.042.64521827
GTCTGAA67450.042.49813517
ACCACTC66650.041.48510732
CACTCAA66750.041.1615434
CTCAATC63050.040.96772436
CACACGT70650.040.72334712
AATCTCG63700.040.54919439
AAAAGGG64900.038.66623370