FastQCFastQC Report
Fri 15 Jun 2018
HW2NGBGX5_n01_zmtarget0523-6597s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW2NGBGX5_n01_zmtarget0523-6597s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10177963
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1514011.487537339249514TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA396570.38963592223709204No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT216090.21231163838972494No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA203810.2002463557786563No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG134010.13166681781020426No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC112780.11080802710719227No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT111410.10946198173445905No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG104530.10270227942467465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC174350.064.2777846
CTCGTAT174550.063.3422444
TATGCCG176750.063.2861748
TCGTATG177450.063.2337645
GTATGCC179800.062.19316547
TATCTCG179750.061.58799741
GTCACAC188400.060.20904529
TCTCGTA186050.059.57746543
ACTGATA190600.059.49630734
AGTCACA192500.058.98121628
TATATCT189000.058.62989439
TCACACT194500.058.23077430
CAGTCAC196200.057.83325627
TGATATA195950.057.78286436
ATATCTC192100.057.70170240
CCGTCTT195500.057.12645752
GATATAT199300.056.8648437
CTGATAT199950.056.76695335
CACACTG199600.056.69058631
ACGTCTG203950.056.49532715