Basic Statistics
Measure | Value |
---|---|
Filename | HW2NGBGX5_n01_zmtarget0523-6597s.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 10177963 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT | 151401 | 1.487537339249514 | TruSeq Adapter, Index 25 (97% over 44bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 39657 | 0.38963592223709204 | No Hit |
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT | 21609 | 0.21231163838972494 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA | 20381 | 0.2002463557786563 | No Hit |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG | 13401 | 0.13166681781020426 | No Hit |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 11278 | 0.11080802710719227 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT | 11141 | 0.10946198173445905 | No Hit |
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG | 10453 | 0.10270227942467465 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 17435 | 0.0 | 64.27778 | 46 |
CTCGTAT | 17455 | 0.0 | 63.34224 | 44 |
TATGCCG | 17675 | 0.0 | 63.28617 | 48 |
TCGTATG | 17745 | 0.0 | 63.23376 | 45 |
GTATGCC | 17980 | 0.0 | 62.193165 | 47 |
TATCTCG | 17975 | 0.0 | 61.587997 | 41 |
GTCACAC | 18840 | 0.0 | 60.209045 | 29 |
TCTCGTA | 18605 | 0.0 | 59.577465 | 43 |
ACTGATA | 19060 | 0.0 | 59.496307 | 34 |
AGTCACA | 19250 | 0.0 | 58.981216 | 28 |
TATATCT | 18900 | 0.0 | 58.629894 | 39 |
TCACACT | 19450 | 0.0 | 58.230774 | 30 |
CAGTCAC | 19620 | 0.0 | 57.833256 | 27 |
TGATATA | 19595 | 0.0 | 57.782864 | 36 |
ATATCTC | 19210 | 0.0 | 57.701702 | 40 |
CCGTCTT | 19550 | 0.0 | 57.126457 | 52 |
GATATAT | 19930 | 0.0 | 56.86484 | 37 |
CTGATAT | 19995 | 0.0 | 56.766953 | 35 |
CACACTG | 19960 | 0.0 | 56.690586 | 31 |
ACGTCTG | 20395 | 0.0 | 56.495327 | 15 |